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Several in the phenotypes offered in Fig. two were being examined during the context of protease-activated receptor activation by thrombin or PAR4-AP. Whilst our data exposed altered outdoors-in signaling in unstimulated platelets, numerous reports have delivered evidence for agonist-induced enhancement of FAK and ERK signaling in platelets and cells expressing the human Pro33 integrin β

Structurally, the Leu33Pro substitution generates a Pro32Pro33 sequence, which can increase the pliability of integrin α

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thirteen activation through PAR4 stimulation is involved in the spreading of WT samples and could dampen constitutively activated Src in KI mice, as proposed by the significant statistical drug by genotype interactions while in the existence of PAR4-AP.

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: raw densitometry values ended up normalized to the standard of WT values for each specialized replicate. Amount of technological replicates, three. All facts were being analyzed using unpaired t

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max in binding experiments, respectively. Statistical Investigation of personal consultant curves was performed by numerous t

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